Philip Kirby's hope was a sizable price at 33/1.
A significant number of ante-post punters have had their bets on the Grand National scratched, following news that Blaklion will miss the Aintree spectacular.
In total, 5% of all bets on the marquee race are now gone, with the former Becher Chase winner now reportedly finished for the season.
The 10-year-old had proved popular with punters prior to the setback, only trailing the likes of red-hot favourite Tiger Roll, Rathvinden, Vintage Clouds and Rock the Kasbah.
In fact, Blaklion has consistently been in the top ten most backed horses for the race across 2019, although ultimately the gamble has been in vain.
Discussing the unfortunate setback, Kirby said: "I'm disappointed, the owners really wanted to have a National runner and we've done everything we can to get him there in one piece.
"It's not something that will stop him forever, but most likely for this season."
The betting remains largely unchanged following the news, with Tiger Roll a commanding 9/2 best price favourite, and as short as 3/1 in places.
Anibale Fly is second at 12/1 best price, with Rathvinden completing the top three at the same odds of 12/1.
Oddschecker spokesperson George Elek: “He might have been a relatively big price but Blaklion is always a popular horse. His withdrawal will hurt some antepost punters, although the top of the market remains unchanged and it could well be a case of how short Tiger Roll goes before the big day.”